The left lateral and median lobes were mobilized and ligated followed by a resection, resulting in a 70% reduction of the liver tissue. Briefly, the abdominal cavity was opened with a longitudinal incision in the linea alba. The PH-2 and PH-5 animals underwent a partial hepatectomy as previously described. The non-hepatectomized NH rats served as a “baseline” reference. LRC was evaluated using: (1) The total number of Ki-67 positive hepatocytes N(Ki-67, liver) evaluated in a stereology-based design (2) the regenerated protein ratio (RPR) and (3) plasma concentration of coagulation factors II, VII, X, prothrombin-proconvertin ratio (PP) before, and 2 or 5 d after hepatectomy.Īfter 16 wk, both STD and HFCD rats were randomly divided into the following groups of nine rats (Figure 1): (1) No hepatectomy before sacrifice (NH) (2) partial hepatectomy (PH), sacrificed two days post-surgery (PH-2) (3) partial hepatectomy, sacrificed five days post-surgery (PH-5). We studied partially hepatectomized, HFCD-fed rats, hypothesizing that rats with HFCD-induced NASH would have decreased LRC, as well as lower expression of genes related to regeneration. To our knowledge, HFCD models have never been used to study the LRC experimentally. Our group has previously studied a high-fat, high-cholesterol diet (HFCD) rat model with features that closely resemble human NASH. In the MCD, CDD, HFD and a high-fat model combined with fructose, decreased as well as normal liver regeneration have been demonstrated. These previous studies of LRC have reported conflicting results, even when the same dietary models were used. These are widely accepted models of NAFLD/NASH, yet the animals lack many of the clinical and/or histopathological features related to human NAFLD/NASH. Previously, LRC has been studied in various rodent models of NAFLD/NASH generally based on the use of the methionine-choline deficiency diet (MCD), choline deficiency diet (CDD), simple high-fat diets (HFD) and the genetic leptin-deficiency model. It has been proposed that NASH livers are more vulnerable to surgical interventions because of decreased liver regeneration capacity (LRC). Epidemiological studies have shown that liver resection is associated with increased morbidity and mortality in patients with NAFLD following liver resections. Surgical resection of the liver tumor remains the gold standard treatment for both HCC and liver metastases from colorectal cancer.
Further, they have an increased risk of other cancers, for example colorectal carcinoma, which often metastasize to the liver. Patients with NAFLD and especially non-alcoholic steatohepatitis (NASH) have a higher risk of developing primary hepatocellular carcinoma (HCC).
Symphonic Game Music Concert - The Symphonic Game Music Concerts (German: Symphonische Spielemusikkonzerte ) are a series of German video game music concerts produced by Thomas Böcker, notable for being the first of their kind out of Japan.The incidence of obesity and non-alcoholic fatty liver disease (NAFLD) is increasing worldwide, affecting approximately one-third of the general population. Sie beschäftigt sich mit dem Verhältnis von Schnitten durch Körpern (und Projektionen) zu den Körpern selbst. Stereologie - (von griechisch stereos = fest, körperlich) im ursprünglichen Wortsinn ist die räumliche Interpretation von Schnitten. The study of its structure includes looking at the organ system from a physiological and… … Wikipedia Neuromorphology - (from Greek νεῦρον, neuron, nerve μορφή, morphé, “form” λογία, logia, “study of”) is the study of nervous system form, shape, and structure. Etymology: 1960–65 … From formal English to slang.phs a branch of science dealing with the determination of the three dimensional structure of objects based on two dimensional views of them
Stereology - noun Any technique used to obtain three dimensional information from two dimensional images See Also: stereologic, stereological, stereologically, stereologist … Wiktionary a branch of science dealing with the determination of the three dimensional structure of objects based on two dimensional views of them. Stereology - /ster ee ol euh jee, stear /, n.
Stereology - noun Etymology: International Scientific Vocabulary Date: 1963 a branch of science concerned with inferring the three dimensional properties of objects or matter ordinarily observed two dimensionally It is an interdisciplinary field that is largely concerned with the three dimensional interpretation of planar sections of materials or tissues. Stereology - (from Greek stereos = solid) was originally defined as the spatial interpretation of sections.